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Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography

Kong Y, Kang SY, Cho SY

Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already...
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody

Cho SY, Kang SY, Kong Y

The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine....
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Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani

Kong Y, Park CY, Kang SY, Cho SY

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf...
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Immunohistochemical localization of 36 and 29 kDa proteins in sparganum

Kim LS, Kong Y, Kang SY, Cho SY

Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized...
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An evaluation of cellophane thick smear technique for mass stool examination

Cho SY, Lee SH, Rim HJ, Seo BS

To evaluate the Kato's cellophane thick smear techcique in detection ability of various helminth ova, authors examined 1,843 nationwidely collected stool specimens by the two methods, formalin-ether technique and cellophane...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Recent demonstration of helminth eggs and larvae from vegetable cultivating soil

Choi DW, Ock MS, Suh JW

A survey on soil samples collected from the vegetable gardens and vinyl houses in the vicinity of Taegu was conducted for the discovery of helminth eggs and larvae from October...
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Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum

Kim CH, Choi WS

Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen...
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Recent patterns of intestinal helminth infections among the residents in Taegu City, Korea

Joo CY

In order to estimate the recent pattens of intestinal helminth infections among the residents in Taege City, Korea, a survey based on discovery of helminth eggs by formalin-ether sedimentation and...
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Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits

Kim SI

This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8...
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Autoradiographic studies on some parasitic helminth

Yoon JS, Lee SH, Seo BS

Direct contact method of autoradiography was utilized in studying the distribution of exogenous C(14)-proline in Clonorchis sinensis, Eurytrema pancreaticum, Hymenolepis diminuta and Dipylidium caninum. The most distinct autoradiogram were obtained...
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Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium

Kong Y, Kang SY, Cho SY, Min DY

Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of...
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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Studies on the metabolism of C(14)-proline in some parasitic helminths

Yoon JS

A comparison of the absorption and incorporation of C(14)-proline into protein by the 7 kinds of helminth parasites is presented. The radioactivity of free amino acid fraction is greater than...
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IgA response in mice infected with Neodiplostomum seoulensis

Huh S, Lee SU, Won MH, Jeong YG, Kwon YH, Choi CS

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7,...
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Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera

Lee SC, Chung YB, Kong Y, Kang SY, Cho SY

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by...
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Demonstration of helminth eggs and larvae from vegetable cultivating soil

Lee JS

In order to clarify the contamination of vegetable gardens with human intestinal helminths, the soil samples were examined for the presence of eggs and larvae during the period from September...
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Effect of flubendazole against Ascaris lumbricoides, Trichocephalus trichiurus and Enterobius vermicularis in infected children

Min DY, Ahn MH, Kim KM

Effectiveness of flubendazole, a newly introduced as an anthelmintic in Korea, was evaluated in 28 ascariasis, 28 whipworm infections and 17 pinworm infections in primary school children. All patients were...
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Heterophyid trematodes (Heterophyopsis continua, Pygidiopsis summa and Heterophyes heterophyes nocens) from domestic cats in Korea

Eom KS, Son SY, Lee JS, Rim HJ

During 8 april 1983 to 9 April 1985, a total of 181 domestic cats (Felis catus domestica Linnaeus) of unknown localities in Korea were purchased at Seoul Chung-ang market, and...
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