- Purification of the Clonorchis Antigen using a Diethylaminoethyl-Sephadex A50 Column
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Kim JH, Blackwell
- KMID: 1094166
- Yonsei Med J.
- 1963 Dec;4(1):43-50.
- doi: 10.3349/ymj.1963.4.1.43
Antigens for the diagnosis of clonorchiasis have been isolated from the adult Clonorchis. Following extracttion with a phosphate-citrate buffer solution, ultrasonic disintegration, and defatting with a chloroform-methanol mixture, the antigens... -
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- Identification of surface antigens of Trichomonas vaginalis
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Woo NS, Min DY, Leem MH, Choi YK
- KMID: 1715493
- Korean J Parasitol.
- 1993 Mar;31(1):37-42.
- doi: 10.3347/kjp.1993.31.1.37
Surface proteins of Trichomonas vaginalis (T. vaginalis) were analyzed to study the antigenic variation. The surface proteins of protozoa were labelled by N-hydroxysuccinimide-biotin (NHS-biotin), the NHS-biotin-labelled proteins were immunoprecipitated... -
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- Protein composition and antigenicity of the tegument from Paragonimus westermani
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Kim SI, Cho SY
- KMID: 1715471
- Korean J Parasitol.
- 1993 Sep;31(3):269-276.
- doi: 10.3347/kjp.1993.31.3.269
To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component... -
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- Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique
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Joo KH, Hong SC, Chung MS, Rim HJ
- KMID: 1720261
- Korean J Parasitol.
- 1989 Mar;27(1):1-7.
- doi: 10.3347/kjp.1989.27.1.1
Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the... -
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- Antigen analysis of Toxoplasma gondii lysate and excretory-secretory materials by enzyme-linked immunoelectrotransfer blot(EITB)
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Ahn MH, Son HJ, Leem MH, Min DY
- KMID: 1715427
- Korean J Parasitol.
- 1994 Dec;32(4):249-257.
- doi: 10.3347/kjp.1994.32.4.249
Recently, the importance of toxoplasmosis is raised as a complication in immunosuppressed or AIDS patients. Our study focused on the identification of a variety of Toxoplasma antigens by immunoblotting. Rabbits... -
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- Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium
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Kong Y, Kang SY, Cho SY, Min DY
- KMID: 1720276
- Korean J Parasitol.
- 1989 Jun;27(2):131-139.
- doi: 10.3347/kjp.1989.27.2.131
Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of... -
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- Identification of surface antigen of Trichomonas vaginalis
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Min DY, Leem MH, Ahn MH, Ryu JS
- KMID: 1715426
- Korean J Parasitol.
- 1994 Dec;32(4):243-248.
- doi: 10.3347/kjp.1994.32.4.243
Plasma membrane proteins of a Korean isolate of Trichomonas vaginalis HY-1 were fractionated for antigen analysis. Homogenates of T. vaginalis were fractionated by the differential centrifugation using sucrose step-gradient method.... -
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- Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies
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Yong TS, Im K, Chung PR
- KMID: 1715554
- Korean J Parasitol.
- 1991 Sep;29(3):293-310.
- doi: 10.3347/kjp.1991.29.3.293
Clonorchis sinensis is a common parasite of man in Korea. Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of... -
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- Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography
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Kong Y, Kang SY, Cho SY
- KMID: 1715566
- Korean J Parasitol.
- 1991 Mar;29(1):1-7.
- doi: 10.3347/kjp.1991.29.1.1
Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already... -
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- ELISA of rat sera infected with Paragonimus iloktsuenensis
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Lim BK, Lee OR, Nam HS
- KMID: 1720308
- Korean J Parasitol.
- 1990 Dec;28(4):207-212.
- doi: 10.3347/kjp.1990.28.4.207
Enzyme-linked immunosorbent assay (ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis (PIA), P. westermani (PWA) and Clonorchis sinensis (CSA). Three crude antigens (PIA, PWA,... -
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- Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody
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Cho SY, Kang SY, Kong Y
- KMID: 1720301
- Korean J Parasitol.
- 1990 Sep;28(3):135-142.
- doi: 10.3347/kjp.1990.28.3.135
The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine.... -
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- Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum
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Kim CH, Choi WS
- KMID: 1715538
- Korean J Parasitol.
- 1991 Dec;29(4):339-353.
- doi: 10.3347/kjp.1991.29.4.339
Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen... -
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- Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice
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Min DY, Shin MH, Choi R
- KMID: 1715532
- Korean J Parasitol.
- 1992 Mar;30(1):43-48.
- doi: 10.3347/kjp.1992.30.1.43
Paragonimus westermani is a common fluke in Korea. The present study aimed to observe the cell mediated immune response in experimental paragonimiasis of mice. The mouse (BALB/c) was orally inoculated... -
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- A study on the body fluid antigen of Clonorchis sinensis using immunogold labeling method
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Chu BD, Rim HJ, Kim SJ
- KMID: 1720294
- Korean J Parasitol.
- 1990 Mar;28(1):11-23.
- doi: 10.3347/kjp.1990.28.1.11
In order to observe the antigenic localization in the tissues of the adult Clonorchis sinensis, immunogold labeling method was applied using serum immunoglobulins (IgG) of either worm-infected rabbits (group I)... -
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- Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera
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Lee SC, Chung YB, Kong Y, Kang SY, Cho SY
- KMID: 1715494
- Korean J Parasitol.
- 1993 Mar;31(1):43-48.
- doi: 10.3347/kjp.1993.31.1.43
Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by... -
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- Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique
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Min DY, Leem MH, Kim JM, Choi YK
- KMID: 1715511
- Korean J Parasitol.
- 1992 Dec;30(4):323-328.
- doi: 10.3347/kjp.1992.30.4.323
Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed... -
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- Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis
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Morakote N, Kong Y
- KMID: 1715487
- Korean J Parasitol.
- 1993 Jun;31(2):169-171.
- doi: 10.3347/kjp.1993.31.2.169
Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,... -
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- TI: Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. II: Protection against natural exposure under field conditions
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Baek BK, Kim BS, Choi IH, Yang KC, Kakoma I
- KMID: 1715522
- Korean J Parasitol.
- 1992 Sep;30(3):201-208.
- doi: 10.3347/kjp.1992.30.3.201
A Theileria sergenti soluble merozoite preparation containing the 29, 34, 35 and 105 KD as the immunodominant polypeptides, was evaluated for efficacy, safety and protectivity in Holstein calves against... -
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- Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method
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Rim HJ, Kim SJ, Sun IJ, Lee JS
- KMID: 1715527
- Korean J Parasitol.
- 1992 Mar;30(1):1-14.
- doi: 10.3347/kjp.1992.30.1.1
In order to observe the antigenic localization in the tissues of Paragonimus westermani of developmental stages, immunogold labeling method was applied using serum of the cats which were infected with... -
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- Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth
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Rim HJ, Kim SJ, Yang MG
- KMID: 1715510
- Korean J Parasitol.
- 1992 Dec;30(4):309-321.
- doi: 10.3347/kjp.1992.30.4.309
In order to observe the antigenic localization in the tissues of Metagonimus yokogawai in growth stages, immunogoldlabeling method was applied to using serum of the cat which infected with isolated... -
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