J Korean Surg Soc.  2007 Jul;73(1):13-20.

Effect of Rapamycin on the Cell Cycle Arrest of T Lymphocytes

• Lee JS
• Han C
• Cho SN
• Chung SY

Abstract

PURPOSE: Rapamycin (RPM) and its analogues are known for their potent immunosuppressant and anti-proliferative properties, which stem from their ability to modulate the signal transduction pathways involved in cell cycle progression from the G1 to S phase. Thus, RPM has been shown to inhibit the proliferation of a number of non-immune cell types, including hepatocytes, vascular smooth cells and fibroblasts. In addition to its effects on proliferation, RPM may also play a role in the regulation of apoptosis under certain circumstances.
METHODS
The effects of RPM on the activation, proliferation and expression of cytotoxic effector molecules were examined on Molt-4 human T-lymphocyte by determining its effects on apoptosis, cell viability, reactive oxygen species (ROS) production and mitochondrial dysfunction. Cells were cultured in the presence or absence of RPM, and then analyzed by Flow cytometry after staining with PI (propidium iodide).
RESULTS
The viability of Molt-4 T cells dose- and time-dependently decreased on the addition of RPM.
CONCLUSION
RPM induced cytotoxicity was characterized by G2/M phase cell cycle arrest. In addition, a pharmacological scavenging study of ROS, including H2O2, revealed the cytotoxicity was mainly induced by the generation of ROS, which might modulate the expression of Bak protein and mitochondrial dysfunction.

Keyword

Rapamycin; Apoptosis; Molt-4 T cell; Cytotoxicity

MeSH Terms

Apoptosis
bcl-2 Homologous Antagonist-Killer Protein
Cell Cycle Checkpoints*
Cell Cycle*
Cell Survival
Fibroblasts
Flow Cytometry
Hepatocytes
Humans
Reactive Oxygen Species
S Phase
Signal Transduction
Sirolimus*
T-Lymphocytes*
Reactive Oxygen Species
Sirolimus
bcl-2 Homologous Antagonist-Killer Protein