J Korean Neurosurg Soc.  2013 Feb;53(2):72-76. 10.3340/jkns.2013.53.2.72.

Dysregulated Expression Profiles of MicroRNAs of Experimentally Induced Cerebral Aneurysms in Rats

Affiliations
  • 1Department of Neurosurgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Korea. yangjiho1963@gmail.com
  • 2Clinical Research Institute, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Korea.

Abstract


OBJECTIVE
Cerebral aneurysm (CA) is an important acquired cerebrovascular disease that can cause catastrophic results. MicroRNAs (miRNAs) are small non-coding RNAs, playing essential roles in modulating basic physiologic and pathological processes. Currently, evidences have been established about biologic relationship between miRNAs and abdominal aortic aneurysms. However, biologic roles of miRNAs in CA formation have not been explained yet. We employed microarray analysis to detect and compare miRNA expression profiles in late stage of CA in rat model.
METHODS
Twenty-six, 7-week-old male Sprague-Dawley rats underwent a CA induction procedure. The control animals (n=11) were fed a normal diet, and the experimental animals (n=26) were fed a normal diet with 1% normal saline for 3 months. Then, the rats were sacrificed, their cerebral arteries were dissected, and the five regions of aneurysmal dilation on the left posterior communicating artery were cut for miRNA microarrays analysis. Six miRNAs (miRNA-1, miRNA-223, miRNA-24-1-5p, miRNA-551b, miRNA-433, and miRNA-489) were randomly chosen for validation using real-time quantitative PCR.
RESULTS
Among a set of differentially expressed miRNAs, 14 miRNAs were over-expressed more than 200% and 6 miRNAs were down-expressed lower than 50% in the CA tissues.
CONCLUSION
The results show that miRNAs might take part in CA formation probably by affecting multiple target genes and signaling pathways. Further investigations to identify the exact roles of these miRNAs in CA formation are required.

Keyword

Intracranial aneurysm; MicroRNAs; Cell proliferation; Apoptosis; Inflammation

MeSH Terms

Aneurysm
Animals
Aortic Aneurysm, Abdominal
Apoptosis
Arteries
Cell Proliferation
Cerebral Arteries
Diet
Humans
Inflammation
Intracranial Aneurysm
Male
Microarray Analysis
MicroRNAs
Pathologic Processes
Rats
Rats, Sprague-Dawley
RNA, Small Untranslated
MicroRNAs
RNA, Small Untranslated

Figure

  • Fig. 1 Cerebral aneurysm is experimentally induced at the left posterior communicating artery in the circle of Willis in a Sprague-Dawley rat (white arrowhead in the magnified view). MCA : middle cerebral artery, ICA : internal carotid artery, BA : basilar artery, PCA : posterior cerebral artery, ACA : anterior cerebral artery, OA : olfactory artery, PcomA : posterior communicating artery.

  • Fig. 2 Hierarchical clustering analysis of microRNA (miRNA) expression of CAs and control cerebral arteries. MiRNAs are presented in rows and samples are presented in columns. Colors indicate relative signal intensities; red and blue colors indicate up-expressed and down-expressed miRNAs, respectively. The rno-miR-24-1* and rno-miR-22* are previous names and changed into rno-miR-24-1-5p and rno-miR-22-5p, respectively.

  • Fig. 3 Summary of real-time qPCR analysis for the up-expressed miRNAs (miR-1, miRNA-223, and miRNA-24-1-5p) and the down-expressed miRNAs (miRNA-551b, miRNA-433, and miRNA-489). Six randomly selected miRNAs are listed on the x-axis, and relative expression levels are placed on the y-axis as up and down direction. *Significant difference between CAs and control arteries (p<0.05). qPCR : quantitative PCR, miRNA : microRNA, CA : cerebral aneurysm.


Cited by  1 articles

Identification of MicroRNAs with Altered Expression Profiles in a Rat Model of Experimentally Induced Early Cerebral Aneurysms
Seung-Hwa Jeong, Hyung-Jin Lee, Jin-Seok Yi, Hong-Jae Lee, Il-Woo Lee, Ki-Cheol Park, Ji-Ho Yang
Korean J Neurotrauma. 2013;9(2):41-46.    doi: 10.13004/kjnt.2013.9.2.41.


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