J Korean Neuropsychiatr Assoc.  2003 Jul;42(4):454-460.

Association Study between Schizophrenia and Monocyte Chemoattractant Protein-1(MCP-1) Gene Polymorphism

• Chung KI
• Pae CU
• Jo JH
• Kim JJ
• Lee CU
• Lee SJ
• Lee C
• Paik IH
• Bang SK

Abstract

OBJECTIVES
This study was aimed at comparing the Monocyte Chemoattractant Protein-1 (MCP-1) gene polymorphic variants of schizophrenics with those of normal controls, and to investigate its contribution to the development of schizophrenia.
METHODS
One hundred and thirty five schizophrenics in accordance with DSM-IV criteria and one hundred and fourteen healthy individuals participated in this study. DNA was extracted from peripheral blood using proteinase K, and the MCP-1 gene promoter region was amplified by polymerase chain reaction (PCR). Gene typing was analyzed by restriction fragment length polymorphism (RFLP) using restriction enzyme PvuII.
RESULTS
Distribution of the genotypes and alleles of MCP-1 gene in the patient group was not significantly different from that of the control group (p=0.145 and p=0.122, respectively). There was difference in the frequencies of genotypes between positive and negative subgroup (p=0.035), but no difference in frequencies of alleles between the two subgroups (p=0.078). There was no difference in scores of PANSS general, positive and negative subscale, the age of onset, the presence or absence of family history between patients with or without -2518G allele (p=0.979, p=0.378, p=0.056, p=0.256 and p=0.301, respectively).
CONCLUSION
We suggest that the polymorphism in the MCP-1 promotor gene may be the possible marker for subgouping of negative type schizophrenia.

Keyword

Schizophrenia; Monocyte chemoattractant protein-1 gene

MeSH Terms

Age of Onset
Alleles
Chemokine CCL2
Diagnostic and Statistical Manual of Mental Disorders
DNA
Endopeptidase K
Genotype
Humans
Monocytes*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Promoter Regions, Genetic
Schizophrenia*
Chemokine CCL2
DNA
Endopeptidase K