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Clin Exp Reprod Med. 2017 Mar;44(1):52-55. English. Case Report. https://doi.org/10.5653/cerm.2017.44.1.52
Chen H , Feng G , Zhang B , Zhou H , Shu J , Gan X .
Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, China. cestbon269@sina.com
Center for Reproductive Medicine, The Obstetrics and Gynecology Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
Abstract

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.

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