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Clin Exp Reprod Med. 2015 Jun;42(2):51-57. English. Original Article.
Lee YS , Park MJ , Park SH , Koo JS , Moon HS , Joo BS .
Department of Pediatric Cardiology, Dong-A University Hospital, Busan, Korea.
Research Center for Anti-Aging Technology Development, Pusan National University, Busan, Korea.
Department of Obstetrics and Gynecology, Good Moonhwa Hospital, Busan, Korea.

OBJECTIVE: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age. METHODS: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 microm. RESULTS: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones. CONCLUSION: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

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