Journal Browser Advanced Search Help
Journal Browser Advanced search HELP
Maxillofac Plast Reconstr Surg. 2016 Nov;38(11):48. English. Original Article. https://doi.org/10.1186/s40902-016-0095-8
Shin SH , Kim KH , Choi NR , Kim IR , Park BS , Kim YD , Kim UK , Kim CH .
Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Beomeo, Mulgeum, Yangsan 626-770, Republic of Korea.
Department of Oral Anatomy and Cell Biology, School of Dentistry, Pusan National University, Beomeo, Mulgeum, Yangsan 626-770, Republic of Korea.
Department of Oral and Maxillofacial Surgery, Dentistry, Dong-A Medical Center, 602-715 Pusan, Republic of Korea.
Abstract

BACKGROUND: This study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser. METHODS: Human fetal osteoblastic (hFOB 1.19) cells were treated with 50 μM alendronate. Then, they were irradiated with a 1.2 J/cm² low-level Ga-Al-As laser (λ = 808 ± 3 nm, 80 mW, and 80 mA; spot size, 1 cm²; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed. RESULTS: In the cells treated with alendronate at concentrations of 50 μM and higher, cell survivability significantly decreased after 48 h (p < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h (p < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT. CONCLUSIONS: The LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.

Copyright © 2019. Korean Association of Medical Journal Editors.