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Ann Lab Med. 2012 Sep;32(5):359-361. English. Brief Communication.
Hong SS , Kim K , Huh JY , Jung B , Kang MS , Hong SG .
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
Department of Laboratory Medicine, CHA Gumi Medical Center, CHA University, Gumi, Korea.

In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-beta-lactamase nonproducers.

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