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Ann Lab Med. 2012 Sep;32(5):359-361. English. Brief Communication. https://doi.org/10.3343/alm.2012.32.5.359
Hong SS , Kim K , Huh JY , Jung B , Kang MS , Hong SG .
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea. hlseo@cha.ac.kr
Department of Laboratory Medicine, CHA Gumi Medical Center, CHA University, Gumi, Korea.
Abstract

In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-beta-lactamase nonproducers.

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