Pasteurellae are aerobic gram-negative coccobacillary organism and usually pathogenic to domestic animal, but rarely to human beings. Human Pasteurella infections are mostly caused by animal but occasionally happens without known history of animal contacts. Pasteurella infection of human has been reported in numerous systems including pneumonia, empyema, meningitis, peritonitis, bone and joint infection and septicemia. This organism was difficult to identify because of its superficial resemblance to other organism, until recently a new technique used a requirement of X, V factor is developed. This organism is susceptible to many antimicrobial agents. A 55-year-old man was admitted to Seoul Paik Hospital Inje University due to fever and pleuritic chest pain for 2 weeks. He denied any animal contact history. Pasteurella hemolytica was recovered from pleural effusion. This is the first case report of empyema caused by Pasteurella hemolytica in Korea and provides another definitive causative organism of empyema.