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World J Mens Health. 2018 Jan;36(1):66-72. English. Original Article. https://doi.org/10.5534/wjmh.17025
Yu HS , Park J , Lee HS , Park SA , Lee DW , Park K .
Department of Urology, Chonnam National University Medical School, Chonnam National University Sexual Medicine Research Center, Gwangju, Korea. uropark@gmail.com
Nano Convergence and Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon, Korea.
MEMS and Nanotechnology Laboratory, School of Mechanical Systems Engineering, Chonnam National University, Gwangju, Korea.
Abstract

Purpose

To investigate the feasibility of a polycaprolactone (PCL) scaffold fabricated by three-dimensional (3D) printing for tissue engineering applications for tunica albuginea.

Materials and Methods

PCL scaffolds were fabricated by use of a 3D printing system. Two scaffolds were fabricated that differed in the architecture of the lay-down pattern: a 90°PCL scaffold and a 45°PCL scaffold. Mechanical properties were measured to compare tensile strength between the two scaffold types. The scaffolds were characterized by scanning electron microscope (SEM) images. The scaffolds were seeded with fibroblast cells, and the ability of these scaffolds to support the cells was evaluated by immunofluorescence staining.

Results

The PCL scaffolds had well-structured shapes, regular arrays, and good interconnection in SEM images. The horizontal and vertical Young's modulus coefficients were 13 and 12 MPa for the 90°PCL scaffold and 19 and 21 MPa for the 45°PCL scaffold, respectively. Microscopy images revealed that human fibroblast cells covered the entire scaffold surface. Immunofluorescence staining of ER-TR7 confirmed that the fibroblast cells remained viable and proliferated throughout the time course of the culture.

Conclusions

This preliminary study provides experimental evidence for the feasibility of 3D printing of PCL scaffolds for tissue engineering applications of tunica albuginea.

Copyright © 2019. Korean Association of Medical Journal Editors.