Journal Browser Advanced Search Help
Journal Browser Advanced search HELP
-
World J Mens Health. 2016 Aug;34(2):137-144. English. Original Article. https://doi.org/10.5534/wjmh.2016.34.2.137
Kim WH , Bae WJ , Park JW , Choi JB , Kim SJ , Cho HJ , Ha US , Hong SH , Lee JY , Hwang SY , Kim SW .
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ksw1227@catholic.ac.kr
Catholic Integrative Medicine Research Institute, The Catholic University of Korea, Seoul, Korea.
Korea Bio Medical Science Institute, Seoul, Korea.
Abstract

PURPOSE: We compared a transperineal ligation model and a transperitoneal ligation model in male rats to determine which animal model of overactive bladder (OAB) was more useful based on cystometrography, estimations of oxidative stress, and measurements of pro-inflammatory cytokine levels. MATERIALS AND METHODS: Male rats were randomly divided into three groups (n=15 in each): the control group, the transperineal ligation group, and the transperitoneal ligation group. Four weeks after the ligation procedure, cystometrography was performed and oxidative stress, pro-inflammatory cytokine levels, and histologic changes were evaluated. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine and superoxide dismutase, and pro-inflammatory cytokine activity was investigated by measuring levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α. RESULTS: The transperineal model led to results similar to those observed for the transperitoneal model, namely (1) increased voiding frequency and reductions in the non-voiding contraction interval and the maximal vesical pressure, (2) increased levels of oxidative stress markers, (3) increased pro-inflammatory cytokine levels, and (4) fibrotic changes in the bladder tissue. CONCLUSIONS: We suggest that the transperineal procedure can be used as an alternative OAB model in male rats.

Copyright © 2019. Korean Association of Medical Journal Editors.