BACKGROUND: Fluorescein angiography is the standard tool for the diagnosis of vitreoretinal diseases. Fluorescein sodium has been widely used for decades. Although its adverse reactions are mostly non-immunologic and mild, fatal reactions, such as anaphylaxis, occur rarely. CASE HISTORY: A 64-year-old woman experienced erythema on her face and neck, dyspnea, hypotension and loss of consciousness immediately after injection of fluorescein for angiography. Her symptoms recovered after intravenous infusion of fluid along with injection of epinephrine, steroid and antihistamine. RESULTS: The skin prick test showed a positive reaction to fluorescein sodium. CONCLUSION: Based on her symptoms and the skin prick test result, the patient was diagnosed with fluorescein-induced anaphylaxis.