PURPOSE: This study was conducted to develop and evaluate the effects of a cancer prevention program for nursing students. METHODS: A nonequivalent control group pretest-posttest design was used. Participants were 53 nursing students (intervention group: 26, control group: 27). The cancer prevention program was provided for 3 weeks (3 sessions, 6 hours). Collected data were analyzed using SPSS/WIN 21.0 program. RESULTS: Compared with the control group, nursing students in the cancer prevention program group showed significant differences in perceived benefits (F=6.98, p=.001), perceived self-efficacy (F=16.00, p<.001), activity related affect (F=14.01, p<.001) and health behavior for cancer prevention (F=16.59, p<.001). CONCLUSION: Study findings indicate that the cancer prevention program for nursing students is effective and can be recommended as an intervention for improving the health behavior of cancer prevention in nursing students.