Amniotic membrane is the innermost layer of the placenta and consists of a thick basement membrane and an avascular stromal matrix. Amniotic membrane transplantation facilitates rapid healing with recovery of a normal epithelial phenotype in the epithelium, and reduces inflammation, vascularization, and scarring in the stroma. Amniotic membrane has two major role, basement membrane and cocktail of cytokines. Amniotic membrane as a native matrix can be used as a graft to restore conjunctival surfaces and corneal surfaces suffering from intractable corneal disease or limbal stem cell deficiency. To restore limbal stem cell deficiency, the source of stem cell has been introduced the autograft transplantation, allograft transplantation, ex vivo expansion and in vivo expansion.