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Int Neurourol J. 2018 Mar;22(1):9-19. English. Original Article. https://doi.org/10.5213/inj.1835052.526
Zhang C , Zhang Y , Cruz Y , Boone TB , Munoz A .
Department of Biomedical Engineering, University of Houston, Houston, TX, USA.
Regenerative Medicine and Tissue Engineering Program-Urology, Houston Methodist Research Institute, Houston, TX, USA. alvaro_munoz@hotmail.com
Guangdong Provincial Work Injury Rehabilitation Hospital, Guangdong, China.
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Department of Urology, Houston Methodist Hospital, Houston, TX, USA.
Instituto de Neurociencias Traslacionales. Universidad de Guadalajara, Jalisco, México.
Abstract

Purpose

To characterize the electromyographic activity of abdominal striated muscles during micturition in urethane-anesthetized female mice, and to quantitatively evaluate the contribution of abdominal responses to efficient voiding.

Methods

Cystometric and multichannel electromyographic recordings were integrated to enable a comprehensive evaluation during micturition in urethane-anesthetized female mice. Four major abdominal muscle domains were evaluated: the external oblique, internal oblique, and superior and inferior rectus abdominis. To further characterize the functionality of the abdominal muscles, pancuronium bromide (25 μg/mL or 50 μg/mL, abdominal surface) was applied as a blocking agent of neuromuscular junctions.

Results

We observed a robust activation of the abdominal muscles during voiding, with a consistent onset/offset concomitant with the bladder pressure threshold. Pancuronium was effective, in a dose-dependent fashion, for partial and complete blockage of abdominal activity. Electromyographic discharges during voiding were significantly inhibited by applying pancuronium. Decreased cystometric parameters were recorded, including the peak pressure, pressure threshold, intercontractile interval, and voiding duration, suggesting that the voiding efficiency was significantly compromised by abdominal muscle relaxation.

Conclusions

The relevance of the abdominal striated musculature for micturition has remained a topic of debate in human physiology. Although the study was performed on anesthetized mice, these results support the existence of synergistic abdominal electromyographic activity facilitating voiding in anesthetized mice. Further, our study presents a rodent model that can be used for future investigations into micturition-related abdominal activity.

Copyright © 2019. Korean Association of Medical Journal Editors.