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Healthc Inform Res. 2016 Jul;22(3):238-242. English. Case Report. https://doi.org/10.4258/hir.2016.22.3.238
Yoon WB , Kim H , Kim KG , Choi Y , Chang HJ , Sohn DK .
Biomedical Engineering Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea. gsgsbal@ncc.re.kr
Molecular Imaging & Therapy Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
Colorectal Cancer Branch, Research Institute and Hospital, National Cancer Center, Goyang, Korea.
Abstract

OBJECTIVES: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. METHODS: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. RESULTS: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. CONCLUSIONS: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.

Copyright © 2019. Korean Association of Medical Journal Editors.