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Infect Chemother. 2017 Jun;49(2):117-122. English. Original Article. https://doi.org/10.3947/ic.2017.49.2.117
Choi EJ , Lee HS , Noh JY , Song JY , Cheong HJ , Shin OS , Lee H , Jeong M , Kim WJ .
BK21 Plus Graduate Program Biomedical Sciences, Korea University College of Medicine, Seoul, Korea. wjkim@korea.ac.kr
Division of Infectious Diseases, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea.
Transgovernmental Enterprise for Pandemic Influenza in Korea, Seoul, Korea.
Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Korea.
Department of Microbiology, Korea University College of Medicine, Seoul, Korea.
GeneOne Life Science, Inc., Seoul, Korea.
Abstract

BACKGROUND: In March 2013, human infection with avian influenza A (H7N9) virus emerged in China, causing serious public health concerns and raising the possibility of avian-source pandemic influenza. Thus, the development of an effective vaccine for preventing and rapidly controlling avian influenza A (H7N9) virus is needed. In this study, we evaluated the immunogenicity of a synthetic DNA vaccine against H7 HA antigens in mice. MATERIALS AND METHODS: The synthetic consensus H7 HA DNA vaccine (25 or 50 µg) was administered to BALB/c mice at 0, 14, and 28 days by intramuscular injection followed by electroporation. Humoral and cellular immune responses were analyzed in a hemagglutination inhibition test and interferon-gamma enzyme-linked immunospot (ELISpot) assay, respectively. RESULTS: H7 HA-vaccinated mice showed 100% seroprotection and seroconversion rate against H7N9 reassortant influenza virus after both second and third immunizations. The geometric mean titer by the hemagglutination inhibition test increased with an increasing number of immunizations. However, there was no significant difference in geometric titer between the two groups injected with 25 and 50 µg of H7 HA DNA vaccine after two (79.98 vs. 107.65, P = 0.39) and three (159.96 vs. 215.28, P = 0.18) doses. In addition, the ELISpot assay revealed that administration of H7 HA DNA vaccine induced potent interferon-gamma production from mouse splenocytes. CONCLUSIONS: This study demonstrated the humoral and cellular immunogenicity of synthetic consensus H7 HA DNA vaccine in mice. This work demonstrates the potential of the H7 HA DNA vaccine as an efficient tool for the rapid control of emerging influenza A (H7N9) virus.

Copyright © 2019. Korean Association of Medical Journal Editors.