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Korean J Hematol. 2007 Jun;42(2):106-113. Korean. Original Article. https://doi.org/10.5045/kjh.2007.42.2.106
Cho HM , Hong JW , Kim JG , Kim JA .
Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea. jakim@catholic.ac.kr
Hematopoietic Stem Cell Transplantation Labaratory, The Catholic University of Korea College of Medicine, Seoul, Korea.
Department of General Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea.
Abstract

BACKGROUND: Angiogenesis is enhanced in the ischemic tissues after the injection of bone marrow cells (BMCs). However the exact mechanisms for this are not yet fully understood. METHODS: A unilateral ischemic limb was surgically induced in mice and then BMCs were injected into the ischemic area. We measured the capillary/muscle ratio. Fluorescence-labeled BMCs were injected into the ischemic tissues and then the locations of the cells were examined by using a confocal microscope. Recruitment of bone marrow-derived cells into the ischemic tissue was examined in a sex-mismatched bone marrow transplantation (BMT) setting by identifying the Y chromosome with using the FISH technique. The expressions of VEGF, MMP-9, SDF-1 and CXCR-4 were measured by Western blot analysis. RESULTS: The capillary/muscle ratio was more increased in the BMC-injected group than in the control group (P<0.05). Florescence-labeled BMCs, which had been directly injected into ischemic tissue, were not detected in the tissue. In the sex-mismatched bone marrow transplantation models, the ischemic tissues of the BMC-injected group recruited a much greater number of Y chromosome-positive bone marrow- derived cells, as compared to the control group. The expressions of VEGF and MMP-9 were increased after injection of BMCs. SDF-1 was expressed on the seventh day in the BMC-injected group and CXCR-4 was highly expressed until 12 weeks in the BMC-injected group. CONCLUSION: We suggest that the injection of BMCs into ischemic tissue recruits CXCR-4-positvie cells from the bone marrow via the up-regulation of VEGF, MMP-9 and SDF-1, and these CXCR-4-positive cells may play a role in neovascularization.

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