Prosthetic valve thrombosis (PVT) can be a life-threatening complication that requires immediate treatment. This is a case report on repeated thrombolytic therapy for thrombosis of a prosthetic tricuspid valve. After repeated urokinase therapy, mechanical clicks were audible and the hemodynamics of the patient rapidly improved. Echocardiography showed the disappearance of thrombi and a normalized pressure gradient. Cine-fluoroscopy showed a well-functioning prosthetic valve.