PURPOSE: To evaluate the usefulness of ultrasound-guided fine-needle aspiration biopsy (FNAB) for salivary gland masses. MATERIALS AND METHODS: Medical records of thirty patients who underwent both ultrasound-guided fine-needle aspiration biopsy and subsequent surgical removal of salivary gland tumors between 2000 and 2002 were retrospectively reviewed. The results of FNABs were compared to the histopathological diagnosis obtained from the surgical specimens. RESULTS: The results of FNAB were concordant with the histopathologic diagnosis in all patients. FNAB showed a high specificity (100%) and positive predictive value (100%) for malignancy whereas it showed a low sensitivity (66.7%). CONCLUSION: Overall, ultrasound-guided FNAB for salivary gland masses was highly useful. However, the low sensitivity of FNAB for malignancy should be taken into consideration, and when pleomorphic adenoma was diagnosed by FNAB, the possibility of malignancy can not be excluded.