Experimental brain tumor model is essential for the development of new therapeutic modalities of brain tumors and evaluation of efficacy of each therapeutic variety. Authors developed experimental rat brain tumor model with 9L and C6 cell line in Fischer rat using stereotactic method. We tried to determine the tumor occurrence rate, the ideal time for secondary experiment using this brain tumor model, and the duration between the onset of neurological signs and the time of expiration. We performed autoradiography for each cell line to evaluate the reliance of the tumor model. We could make good tumor model in all the cases of experiment and except to use it in another extension of experiment.