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J Korean Acad Conserv Dent. 2009 May;34(3):192-198. English. In Vitro.
Kang MK , Bae IH , Koh JT , Hwang YC , Hwang IN , Oh WM .
Department of Conservative Dentistry, School of Dentistry, Chonnam National University, Korea.
Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Korea.
DSRI, Chonnam National University, Korea.
2nd stage of BK21, Chonnam National University, Korea.

This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.

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