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J Korean Soc Osteoporos. 2010 Aug;8(2):178-187. Korean. Original Article.
Lee DY , Kim H , Ku SY , Suh CS , Kim SH , Choi YM , Kim JG .
Department of Obstetrics and Gynecology, Samsung Medical Center, Seoul, Korea.
Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea. kimjg@snu.ac.kr
Department of Obstetrics and Gynecology, Incheon Medical Center, Incheon, Korea.
Abstract

OBJECTIVES: To investigate the relationship between single nucleotide polymorphisms (SNPs) in Wnt signal pathway genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-kappaB ligand (sRANKL) by whole blood cells in postmenopausal Korean women. METHODS: Low density lipoprotein receptor-related protein (LRP) 5 c266 A>G and c3893 C>T, frizzled (FZD) 6 c1033 A>C, axin II c148 C>T, T cell factor (Tcf) 1 c766 G>A, and adenomatous polyposis coli (APC) c5465 T>A polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 141 postmenopausal Korean women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured. RESULTS: The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells, and their ratio of RANKLx1,000/OPG were not different according to the LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms. No significant differences in the production of OPG and sRANKL, and their ratio of RANKLx1,000/OPG were noted among combined genotypes of LRP c266 A>G and c3893 C>T polymorphisms. CONCLUSION: LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms do not affect the production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells.

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