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Int J Oral Biol. 2017 Sep;42(3):137-142. Korean. Original Article. https://doi.org/10.11620/IJOB.2017.42.3.137
Kim JH , Kim AR , Choi YH , Lee DE , Woo GH , Bak EJ , Yoo YJ .
Department of Dental Hygiene, Jeonju kijeon College, Jeonju, Republic of Korea.
Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea. yu618@yuhs.ac, ejbak@yuhs.ac
Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, South Korea.
BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Department of Clinical Laboratory Science, Semyung University, Jecheon, Republic of Korea.
Abstract

To determine the effect of the tumor necrosis factor-α (TNF-α) in odontoclast formation, we administrated a TNF-α inhibitor in rats with diabetes rats with periodontitis. The rats included in the study were divided into three groups: control rats without diabetes or periodontitis (the C group), rats with periodontitis and diabetes (the PD group), and rats with periodontitis and diabetes treated by infliximab, the TNF inhibitor (the PD+infliximab group). The PD and PD+ infliximab groups received intravenous administrations of streptozotocin (STZ, 50 mg/kg) to induce diabetes. After 7 days of STZ injections, the mandibular first molars were ligatured to induce periodontitis. The PD+infliximab group was intrapenitoneally administrated by infliximab (5 mg/kg). On days 3 and 20 after the ligature administration, odontoclast formation along root surfaces was evaluated by tartrate resistant acid phosphatase (TRAP) staining and cathepsin K immunohistochemistry. On day 3, the number of TRAP- and cathepsin K-positive cells increased more so in the PD group than in the C group. The PD+infliximab group showed a lower number of positive cells than the PD group. There was no difference in all the groups on day 20. On day 3, the cathepsin-K positive multinucleated and mononucleated cells were higher in the PD group than in the C group. The number of cathepsin-K positive multinucleated cells was lower in the PD+infliximab group than in the PD group. The PD group showed more cathepsin K-positive cells in the furcation and distal surfaces than the c group. The Cathepsin K-positive cells of the PD+infliximab group were lower than that of the PD group in furcation. These results suggest that TNF-α stimulates odontoclast formation in diabetes with periodontitis.

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