Journal Browser Advanced Search Help
Journal Browser Advanced search HELP
Int J Oral Biol. 2016 Dec;41(4):231-236. Korean. Original Article. https://doi.org/10.11620/IJOB.2016.41.4.231
Park SY , Moon SA , An JM , Kim DS , Seo JT .
Department of Oral Biology, Yonsei University College of Dentistry, Seoul 120-752, Korea. jeong@yuhs.ac
Abstract

Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.

Copyright © 2019. Korean Association of Medical Journal Editors.