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Int J Oral Biol. 2016 Dec;41(4):183-190. Korean. Original Article. https://doi.org/10.11620/IJOB.2016.41.4.183
Kim WG , Lee SA , Moon SM , Kim JS , Kim SG , Shin YK , Kim DK , Kim CS .
Department of Oral and Maxillofacial Surgery, College of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea.
Department of Oral Biochemistry, College of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea. address:cskim2@chosun.ac.kr
Oral Biology Research Institute, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Republic of Korea.
School of integrated Oriental Medical Bioscience, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk, 27136, Republic of Korea.
Abstract

Anthricin (Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer properties in several cancer cells, such as prostate cancer, cervical carcinoma and pancreatic cancer. However, the effects of Anthricin are currently unknown in oral cancer. We examined the anti-cancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner (IC50 50 nM) in the MTT assay and Live & Dead assay. In addition, Anthricin treated FaDu cells showed marked apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factors such as caspase-3, -9 and poly (ADP-ribose) polymerase (PARP), suggesting that caspase-mediated pathways are involved in Anthricin-induced apoptosis. Anthricin treatment also leads to accumulation of the pro-apoptotic factor Bax, followed by inhibition of cell growth. Taken together, these results indicate that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells is mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as a novel chemotherapeutic drug for human oral cancer.

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