Two trials of external quality assessment for conventional newborn screening tests for phenylketonuria, congenital hypothyroidism, galactosemia, maple syrup urine disease and homocytinuria and for the newborn screening tests using tandem mass spectrometry (MS/MS) were performed in 2009. Total 20 specimens were distributed to 13 laboratories. All the control materials were send as dried blood spots. The response rate was 100%. The mean, SD, CV, median and range were analyzed. In addition, two trials of external quality assessment for glycohemoglobin (HbA1c) were performed in 2009. Total 6 samples were distributed to 196 laboratories and the response rate was 97.2% (175/180) and 98.5% (193/196) in each trial. From this trial, we performed accuracy-based survey which used challenge specimens that were free from matrix effects and had target values traceable to certified reference material. Bias between each participants result and reference target value were evaluated.