BACKGROUND: Many reagents have been developed along with advances in chemistry auto analyzer. Deciding on an appropriate reagent is required for an accurate test, diagnosis and efficient laboratory management. We evaluated Cica Liquid reagent produced by Kanto chemical corporation (Tokyo, Japan) for checking reagent ability. METHODS: Twelve chemistry reagents (AST, ALT, ALP, glucose, BUN, creatinine, total bilirubin, direct bilirubin, cholesterol, triglyceride, iron, magnesium) were tested on precision, linearity, interference, and correlation. We have evaluated using Hitachi 7600 (Hitachi High Technologies co., Japan) chemistry auto analyzer in accordance with the CLSI guidelines EP5-A, EP6-A, EP7-A, EP9-A2. EP_Suite (Marchem Associates Inc., USA) and SPSS ver. 11.0 (SPSS Inc., USA) were used for statistics. RESULTS: Precision results were satisfactory to CLIA '88 in all of the analytes except for ALP and magnesium. The linearity was satisfactory in measurement ranges as all analytes showed linearity in polynomial regression result or relative nonlinearity of less than 2.5%. Coefficients of correlation were above 0.985 in all analytes except for direct bilirubin and magnesium. When interference test results were compared with criteria of CLIA '88, low level of AST was positively interfered by hemoglobin and magnesium was negatively interfered by bilirubin. CONCLUSIONS: In conclusion, the Kanto Cica Liquid reagents are valuable in clinical laboratory, since they showed good precision, linearity, and correlation with other reagents.