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Korean J Gynecol Oncol. 2005 Dec;16(4):347-353. Korean. Original Article. https://doi.org/10.3802/kjgo.2005.16.4.347
Kim HK , Kang YH , Kwak SY , Ding GH , Bae SM , Park EK , Lee YS , Kim J , Kim YW , Ro DY , Lee JM , Namkoong SE , Chang HS , Chun HJ , Lim DS , Ahn WS .
Department of Obsterics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ahnws@catholic.ac.kr
Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Department of Therapeutic Radiology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract

OBJECTIVE: The chemotherapeutic agent cisplatin (cis-diamminedichloroplatinum (II)) is particularly effective against cervical cancer. The purpose of this study is to elucidate combination effect of cisplatin and green tea extracts on the growth inhibition of TC-1 cell. METHODS: To observe the anti-proliferative effects, we treated different doses of cisplatin (0.1, 0.5, 2.5 uM), GTP (1, 5, 25 ug/ml) and EGCG (25, 50, 100 uM). to TC-1 cells. Also, we treated 0.5 uM of cisplatin and different doses of GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM). Cell viability was scored by use of MTT assay. In addition, E6 gene expression patterns in TC-1 cell were investigated by using RT-PCR. RESULTS: Cell growth inhibition in a dose dependent was observed at the different concentration of ciaplatin, GTP and EGCG. Also, in the groups treated by 0.5 uM of cisplatin and GTP (1 and 5 ug/ml) or EGCG (25 and 50 uM), the inhibition of cell growth showed with 12.2%, 6.9% and 63.4%, 72.2% as compared to the group treated by cisplatin only. In RT-PCR, down regulation of E6 was shown. CONCLUSION: Additive effect of the combination of cisplatin with GTP or EGCG on the inhibition of cell growth was observed. This effect suggests the possibility lowering the concentration of chemotherapeutic drugs, which alleviate the side effect of drugs.

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