PURPOSE: The purpose of this study was to evaluate the effect of the sexual education program on female college students' sexual knowledge and sexual autonomy. METHODS: The integrative 5-week (15 hour) sexual education program reflecting control and coping aspects of sexual autonomy was developed based on self-determination theory. 59 female students were assigned to the experimental (n=29) and the control group (n=30). Students completed the sexual knowledge and the sexual autonomy questionnaires at prior to the program, right after and 10 weeks after the completion of the program. Independent t-test were used to test the effectiveness of the program. RESULTS: Significant group differences were found on sexual autonomy score right after and 10weeks after the completion of the program. No significant group differences were found on sexual knowledge at any time after the program completion. CONCLUSION: The results indicate that the 5-week Sexual Education Program was effective in increasing female college students' sexual autonomy. Nursing professors majoring women's health nursing may contribute to improving college students' sexual autonomy by opening the sexual education program as a general elective curriculum. Strategies to improve sexual knowledge need to be considered for better effectiveness of the program.