PURPOSE: The purpose of this study was to investigate the relationship of Yangsaeng and HRQOL in middle aged women. Yangsaeng as a traditional health care regimen for the promotion of health and prevention of illness by means of specific principles and methods, which purpose was to improve longevity and healthy life. METHODS: The subjects of this study were 200 middle aged women in Korea. Data were collected by using a self-reported questionnaire and analyzed through descriptive statistics, Pearson's correlation coefficient, t-test, ANOVA and multiple linear regression. RESULTS: There was statistically significant difference in Yangsaeng according to age, stress and working hours. There were significant differences in HRQOL according to stress and working hours. The relationship between Yangsaeng and HRQOL had a significant positive correlation. Mind and diet Yangsaeng, christianity, and working hours were found to be significant predictors (24.6%) of HRQOL. CONCLUSION: To promote HRQOL of middle aged women, the nurse should focus on the factors identified in this study when she develop nursing intervention programs for health promotion.