PURPOSE: To identify the effects of a training program on the knowledge of, attitudes towards child infection, and infection prevention behaviors in staff of daycare centers. METHODS: A nonequivalent control group pretest-posttest design study was conducted with 34 staff of 6 daycare centers over 3 months. The staff in the 3 centers designated as the experimental centers received the training program weekly for 8 weeks. The program included on-site education for one and half hours and monitoring of infection prevention behaviors. Knowledge and attitudes of the staff were measured, and their infection prevention behaviors were observed and recorded by research assistants before and after the program. chi2-test, t-test, Fisher's exact test, and Mann-Whitney U-test with SPSS Win program were used to analyze the data. RESULTS: The group of staff who received the training program recorded higher scores in knowledge, attitudes, and their infection prevention behaviors compared with staff in the control group. CONCLUSION: This finding suggests that the training program had a significant impact on knowledge, attitude, and infection prevention behaviors of staff which could prevent child infection in daycare centers. Nurses need to be involved in daycare centers for the health of the children, and they could intervene effectively in child infections by using this program.