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Korean J Pediatr. 2005 Aug;48(8):894-900. Korean. Original Article.
Kim SK , Ghil HY , Song SU , Choi JW , Park SK .
Hematology-Oncology, Inha University of Hospital, Inha Medical College, Incheon, Korea.
Department of Pediatrics, Inha University of Hospital, Inha Medical College, Incheon, Korea.
Clinical Research Center, Inha University of Hospital, Inha Medical College, Incheon, Korea.
Department of Laboratory Medicine, Inha University of Hospital, Inha Medical College, Incheon, Korea.
Department of Pediatrics, College of Medicine, Ulsan University, Ulsan, Korea. sang@uuh.ulsan.kr
Abstract

PURPOSE: Because of the unavailability of marrow transplantation, umbilical cord blood (CB) is increasingly being used. We evaluated the potential of ex vivo expansion and clonality in CD34+ cells separated from cord blood source and mobilized peripheral blood (PB) in a serum-free media. METHODS: The CD34+ cells, selected from CB and mobilized PB, were expanded with hematopoietic growth factors. They were then cultured for burst-forming units of erythrocytes (BFU-E), colony-forming units of granulocytes and monocytes (CFU-GM) and colony-forming units of megakaryocytes (CFU-Mk) at culture days 0, day 4, day 7, and day 14 with various growth factors. RESULTS: The CB-selected CD34+ cells showed significantly higher total cell expansion than those from the PB at day 7 (2 fold increase than PB). The CB-selected CD34+ cells produced more BFU-E colonies than did the PB on culture at days 7 and at day 14. Also, the CB-selected CD34+ cells produced more CFU-Mk colonies than did the PB on culture at day 4 and at day 7. CONCLUSION: The ex vivo expansion of the CB cells may be promising in producing total cellular expansion, CFU-Mk and BFU-E compared with PB for 7 to 14 days. The growth factors combination including megakaryocyte growth and development, flt3-ligand and interleukin-3 showed more expansion in the view of total cells and clonal maintenance compared with less combination.

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