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Korean J Lab Med. 2011 Oct;31(4):282-284. English. Brief Communication. https://doi.org/10.3343/kjlm.2011.31.4.282
Jang MH , Choi GE , Shin BM , Lee SH , Kim SR , Chang CL , Kim JM .
Department of Laboratory Medicine, School of Medicine, Pusan National University, Yangsan, Korea. cchl@pusan.ac.kr
Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University, Seoul, Korea.
Department of Laboratory Medicine, University of Ulsan College of Medicine Ulsan University Hospital, Ulsan, Korea.
Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea. jmkim@dau.ac.kr
Abstract

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab(TM) system (bioMerieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.

Copyright © 2019. Korean Association of Medical Journal Editors.