PURPOSE: The authors performed this experimental study to test the efficacy and possibility of using a bovine pericardial patch (BPP) with Histoacryl(R) glue for the repair of full-thickness defects in the alimentary tract wall of a mouse model. METHODS: The animal study was conducted under aseptic conditions using 20 rats (4-week-old, 250~300 g each). Rats with a BPP with Histoacryl(R) glue were used to evaluate the effectiveness, strength, adhesion formation, morphological changes, early complications and changes of the BPP character. We made a tube--like form with BPP and Histoacryl(R); we put it in saline (NaCl 0.9%), gastric juices and bile for 2 weeks to test the changes in the material and the strength of the attachment. We tested the BPP with glue attached to the full-thickness defects in the alimentary tract wall. After 3 weeks we scarified the mice and analyzed the patch attachment and microscopic finding. RESULTS: The attached BPP with Histoacryl(R) was put in saline (NaCl 0.9%), gastric juices and bile for 2 weeks, and there were no changes. The attached BPP with glue to the liver surface remained secure. There were no patch failures. We found just one significant adhesion with fibrosis in the surround tissue. But all the other animals mucosal healing was complete in 3 weeks. CONCLUSIONS: BPP with Histoacryl(R) glue may be an acceptable membrane for at least temporary repair of full-thickness defects in the alimentary tract wall. Further study is required.