PURPOSE: The new prosthesis was introduced for female recently considering anthropometric differences of distal femur between male and female. We evaluated the anthropometric measurements of distal femur and compatibility of gender knee prosthesis in total knee replacement . MATERIALS AND METHODS: The candidates were 100 female patients who underwent total knee replacement. We measured the size of resection site which was divided into 6 areas after resection of femur. According to this result, we made a decision which prosthesis would be better fit. Group 1 was whom gender knee prosthesis was used in and group 2 was whom conventional knee prosthesis was used in. RESULTS: Gender knee prosthesis was used in 58cases out of 100 cases (58%). There were significant differences in measurements between group 1 and 2 except anterior flange lengths (p<0.05). Gender knee prosthesis was chosen in 33.4% of size C, in 57.4% of size D, in 70.4% of size E. There were less than 5% discrepancies of width between components and femur. The larger the femur, the lesser the ML/AP ratio. CONCLUSION: Conventional knee prosthesis is adequate to who has smaller distal femur and gender knee prosthesis is adequate to who has larger distal femur in total knee replacement. It is thought that we may select gender knee prosthesis considering the size of femur intraoperatively.