PURPOSE: To determine the prognostic significance of p53 mutations in advanced supraglottic cancer patients. MATERIALS AND METHODS: Twenty-six patients with pertinent tissue materials among 60 patients diagnosed as advanced supraglottic cancer in Kyung Hee university hospital and received total or partial laryngectomy followed by radiation therapy were enrolled. Immunohistochemical staining using DO7 monoclonal antibody was performed. Tumor specimens were analyzed for p53 mutations in exons 5 through 8 by using PCR-SSCP analysis followed by DNA sequencing of all variants. RESULTS: p53 mutations were present in 8 cases among 26 patiets. Mutations within exon 5 were 3 cases, exon 6 were 4 cases, and exon 7 was 1 case. Mean survival time was 70.2 months in patients without mutations, 61.3 months with mutations but there was no statistically significant differences (p=0.596). Mutations were 25% in stage III and 36% in stage IV but there was no statistically significant differences (p=0.563). Mutations were 25% in lymph node negative group and 42% in lymph node positive group but there was no statistically significant differences (p=0.437). CONCLUSION: The presence of a p53 mutation detected by PCR-SSCP is not associated with survival, stage and lymph node status.