Exp Mol Med.  2000 Dec;32(4):243-245.

Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry

• Yon CS
• Han JS

Abstract

For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.

Keyword

GC-MS; phosphatidylethanol; trimethylsilyl derivative; BSTFA

MeSH Terms

Glycerophospholipids/*chemistry
Mass Fragmentography/*methods
Support, Non-U.S. Gov't
Trimethylsilyl Compounds/*chemistry