Yonsei Med J.  1974 Dec;15(2):136-146. 10.3349/ymj.1974.15.2.136.

Comparison of Thioglycollate Media, Modified Thioglycollage Media, and GAM for the Cultivation of Non-Sporeforming Anaerobes

• Chong Y
• Lee SY

• KMID: 687691
• DOI: http://doi.org/10.3349/ymj.1974.15.2.136

Abstract

The clinical bacteriology laboratory has to be prepared to isolate and identify anaerobes as the implication of anaerobes in clinical infections is increasing. Although many types of thioglycollate media have been widely used for the enrichment growth of anerobes, different types are known to have different growth supporting ability. GAM is a recently developed medium, which is said to support a good growth of anaerobes. This study was made to compare GAM and the commonly used thioglycollate medium. It was found that BTM was superior to FTM, but GAM was showing the heaviest growth after a short incubation time. Hemoglobin powder added to FTM or BTM greatly improved growth of Bacteroides without impairing the clarity of the media. Supplementation of FTM with 1/4 strength each of BHI and TSB, and 1000 mg of hemoglobin per liter of medium improved growth of anaerobes. Among all of the tested media, GAM gave the best results for the cultivation of anaerobes including Bacteroides and Fusobacterium.

MeSH Terms

Anaerobiosis
Bacteria/growth & development*
Comparative Study
Culture Media*
Hemoglobins
Thioglycolates*