World J Mens Health.  2025 Apr;43(2):291-303. 10.5534/wjmh.240072.

Adverse Effects of Nicotine on Human Sperm Nuclear Proteins

• Firouzabadi AM
• Henkel R
• Niaki MT
• Fesahat F

• KMID: 2565971
• DOI: http://doi.org/10.5534/wjmh.240072

Abstract

The effects of smoking on human health have long been documented. However, only a few studies have highlighted the direct effects of nicotine on sperm function. Nicotine, as a chemical compound found in tobacco, has been shown to modulate different aspects of spermatogenesis and sperm functions. Nicotine can lead to a reduction in the number of sperm, their motility and functionality. It can change the molecular expressions involved in sperm function, including genes encoding sperm nuclear proteins. The most important nuclear proteins that play a critical role in sperm function are known as H2B histone family, member W, testis-specific (H2BFWT), transition protein 1 (TNP1), transition protein 2 (TNP2), protamine-1 (PRM1), and protamine-2 (PRM2). These proteins are involved in sperm chromatin condensation, which in turn affects fertilization and embryonic development. Any alteration in the expression of these genes due to nicotine exposure/usage may lead to adverse implications in couples’ fertility and the health of future generations. Since research in this area is still relatively new, it underscores the importance of understanding the potential side effects of environmental factors such as nicotine on reproductive health.

Keyword

Fertility; Nicotine; Nuclear proteins; Protamines