Nat Prod Sci.  2024 Dec;30(4):304-308. 10.20307/nps.2024.30.4.304.

Alkaloids with Acetylcholinesterase Inhibitory Activities from Crinum latifolium L.

• Trang VT
• Cong PV
• Dan NV
• Hien NTT
• Tuan DT
• Anh LT
• Thang HD
• Duc NV
• Anh HLT

• KMID: 2564144
• DOI: http://doi.org/10.20307/nps.2024.30.4.304

Abstract

A new crinane-type alkaloid, 6-epihydroxypowelline (1), together with six known alkaloids, lycorine (2), 2-O-acetyllycorine (3), deacetylbowdensine (4), 1-epideacetylbowdensine (5), 8-demethyl-3-oxomaritidine (6), and (-)-marithamine (7) were isolated from the whole parts of the Crinum latifolium L. in Vietnam. The structure identification of all compounds was determined by 1D, 2D-NMR as well as HR-ESI-MS spectroscopic techniques. The absolute configuration of these compounds was established by the ECD data. In addition, in vitro inhibition of acetylcholinesterase (AChE) activities was assessed for all isolated alkaloids. All alkaloids had AChE inhibitory effects, with IC₅₀ values ranging from 32.65 ± 2.72 to 212.76 ± 8.30 µM and compound 3 displayed the strongest inhibition of AChE, with IC₅₀ values of 32.65 ± 2.72 µM (in comparison to the reference drug, galanthamine, which had an IC₅₀ of 2.40 ± 0.45 µM).

Keyword

Crinum latifolium L.; Alkaloids; Acetylcholinesterase inhibitor; Alzheimer’s disease