Int Neurourol J.
2024 Dec;28(4):253-263. 10.5213/inj.2448326.163.
Effect of Eugenol on Detrusor Muscle: Potential for Overactive Bladder Treatment
- Araújo MC
1 - Pereira-Gonçalves 2,3
- Cardeal dos Santos AN1
- Freire JEdC4
- Peireira-de-Morais L5
- Henrique-Félix FS1
- Sousa-Júlio NCC1
- Leal-Cardoso JH3
- Coelho-de-Souza AN1
- Affiliations
-
- 1Experimental Physiology Laboratory, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Brazil
- 2Faculty of Education and Integrated Science, State University of Ceará, Crateús, Brazil
- 3Electrophysiology Laboratory, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Brazil
- 4Laboratory of Biochemistry and Gene Expression, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Brazil
- 5Department of Biological Chemistry, Regional University of Cariri, Crato, Brazil
- KMID: 2562778
- DOI: http://doi.org/10.5213/inj.2448326.163
Abstract
- Purpose
This investigation was conducted to elucidate the effects of eugenol on bladder contractility through experimental and in silico approaches.
Methods
To assess the impact of eugenol on muscular contractility, longitudinal strips of bladder tissue, measuring 2 mm by 6 mm, were mounted in perfusion chambers connected to an isometric force transducer. Furthermore, molecular docking studies were conducted to explore the potential of eugenol to target the M3 muscarinic acetylcholine receptor (M3R) and voltage-operated calcium channels (VOCCs) in muscle cells, utilizing in silico techniques.
Results
Eugenol exhibited a concentration-dependent inhibitory effect on both the phasic and tonic components of the contraction induced by 60mM K+ and carbachol, completely suppressing this contraction at a concentration of 3mM. Additionally, eugenol inhibited the concentration-contraction curve elicited by Ba2+.
Conclusions
The in vitro and in silico results suggest that the mechanism of eugenol likely involves blockade of VOCCs and/or M3R, implicating eugenol as a promising molecule for the treatment of overactive bladder.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Medical Treatment of Overactive Bladder
- The Role of Intra-abdominal Pressure Measurement in Awake Rat Cystometry
- Bladder Outlet Obstruction in the Female Overactive Bladder: Correct Diagnostic Criteria for Bladder Outlet Obstruction?
- The Usefulness of Ice-water Test in the Patients with Symptomatic Benign Prostatic Hyperplasia (BPH) Associated with Overactive Bladder
- Physiologic and Pathophysiologic Functions of Purinoceptors in Voiding Reflex
