Nat Prod Sci.  2024 Jun;30(2):161-166. 10.20307/nps.2024.30.2.161.

Isolation and Identification of Antifungal Metabolites from Juncus torreyi

• Park SH
• Kang JW
• Park GH
• Jo SJ
• Min KW
• Lee KS
• Park HB

• KMID: 2557844
• DOI: http://doi.org/10.20307/nps.2024.30.2.161

Abstract

Plants belonging to the genus Juncus are widely distributed across North America, which are known to make a diverse array of bioactive natural products. In 2022, Juncus torreyi, a species of Juncus, was firstly found in Korea. Morphological and ecological characteristics of the species have been previously investigated; however, bioactive chemical potentials still remain to be explored. In the present work, we focused on the isolation and characterization of metabolites that harbor growth inhibitory activity against a fungal indicator, Candida albicans. Using activity-guided discovery method, we subsequently isolated and purified three metabolites from the most active methylene chloride-soluble fraction. Through NMR and high-resolution ESIOrbitrap-MS data analysis, the metabolites were structurally determined to be juncatrin B (1), ensifolin I (2), and juncusol (3). Metabolites 1–3 were evaluated for their C. albicans growth inhibitory activity and revealed inhibition with an IC 50 value of 74.3, 31.5, and 64.6 μg/mL, respectively.

Keyword

Plant; Natural Products; Secondary Metabolite; Juncus; Phenanthrene