Int J Stem Cells.  2024 May;17(2):158-181. 10.15283/ijsc24056.

Guidelines for Manufacturing and Application of Organoids: Brain

• Kwak T
• Park SH
• Lee S
• Shin Y
• Yoon KJ
• Cho SW
• Park JC
• Yang SH
• Cho H
• Im HI
• Ahn SJ
• Sun W
• Yang JH

• KMID: 2556235
• DOI: http://doi.org/10.15283/ijsc24056

Abstract

This study offers a comprehensive overview of brain organoids for researchers. It combines expert opinions with technical summaries on organoid definitions, characteristics, culture methods, and quality control. This approach aims to enhance the utilization of brain organoids in research. Brain organoids, as three-dimensional human cell models mimicking the nervous system, hold immense promise for studying the human brain. They offer advantages over traditional methods, replicating anatomical structures, physiological features, and complex neuronal networks. Additionally, brain organoids can model nervous system development and interactions between cell types and the microenvironment. By providing a foundation for utilizing the most human-relevant tissue models, this work empowers researchers to overcome limitations of two-dimensional cultures and conduct advanced disease modeling research.

Keyword

Organoid; Brain organoid; Neural organoid; Guideline

Figure

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  Fig. 1 Techniques to establish region-specific brain organoids.

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  Fig. 2 Method for brain organoids specification.

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  Fig. 3 Method for dorsal, ventral forebrain and hypothalamus specification.

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  Fig. 4 Method for pituitary gland, midbrain and cerebellar organoids specification.

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  Fig. 5 Diagram of culture using a 96-well plate.

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  Fig. 6 Cellular composition of developing cerebral cortex.

Reference

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