Int J Stem Cells.  2024 May;17(2):147-157. 10.15283/ijsc24041.

Guidelines for Manufacturing and Application of Organoids: Lung

• Lim K
• Lee MO
• Choi J
• Kim JH
• Kim EM
• Woo CG
• Chung C
• Cho YH
• Hong SH
• Cho YJ
• Ahn SJ

• KMID: 2556234
• DOI: http://doi.org/10.15283/ijsc24041

Abstract

The objective of standard guideline for utilization of human lung organoids is to provide the basic guidelines required for the manufacture, culture, and quality control of the lung organoids for use in non-clinical efficacy and inhalation toxicity assessments of the respiratory system. As a first step towards the utilization of human lung organoids, the current guideline provides basic, minimal standards that can promote development of alternative testing methods, and can be referenced not only for research, clinical, or commercial uses, but also by experts and researchers at regulatory institutions when assessing safety and efficacy.

Keyword

Organoid Standards Initiative; Human lung; Lung organoid

Figure

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  Fig. 1 A schematic diagram outlining the workflow for producing human lung organoids from adult lungs and pluripotent stem cells. In this process, alveolar type 2 (AT2) cells and basal cells from adult human lungs serve as sources of stem/progenitor cells capable of forming alveolar and airway lineage organoids, demonstrating differentiation potential towards their respective downstream cell types. Pluripotent stem cells also can be utilized as a source of cells, giving rise to lung lineage cell type via definitive endoderm, anterior foregut endoderm, and essentially NKX2.1＋ lung progenitor cells. Under suitable culture condition, the NKX2.1＋ lung progenitor cells can differentiate to alveolar, or airway lineage committed organoids. BioRender.com was used for creating this diagram. NGFR: nerve growth factor receptor, ESC: embryonic stem cell, iPSC: induced pluripotent stem cell.

Reference

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