Restor Dent Endod.  2024 Feb;49(1):e9. 10.5395/rde.2024.49.e9.

Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging

• Cortez TV
• Cerqueira NM
• Gallas JA
• Oliveira WP
• Corona SAM
• Souza-Gabriel AE

• KMID: 2554137
• DOI: http://doi.org/10.5395/rde.2024.49.e9

Abstract

Objectives
This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology.
Materials and Methods
The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months.
Results
The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags.
Conclusions
The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin.

Keyword

Antioxidant; Dentin-bonding agent; Punica granatum

Figure

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  Figure 1 Failure modes after immediate bonding test and adhesive interface aging.SBS, shear bond strength.

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  Figure 2 SEM of the interface of the restorative procedure (no degradation after the restorative procedure). (A) Sound dentin (control), (B) sound dentin + 10% P. granatum, (C) eroded dentin, and (D) eroded dentin + 10% P. granatum. Demineralized dentin without resin tags (red asterisk).R, resin; A, adhesive; HL, hybrid layer; D, dentin; SEM, scanning electron microscopy.

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  Figure 3 SEM of the interface after degradation of the adhesive interface (12 months). (A) Sound dentin (control), (B) sound dentin + 10% P. granatum, (C) eroded dentin, (D) eroded dentin + P. granatum 10%. Demineralized dentin without resin tags (red asterisk).R, resin; A, adhesive; HL, hybrid layer; D, dentin; SEM, scanning electron microscopy.

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