Ann Lab Med.  2023 Nov;43(6):632-634. 10.3343/alm.2023.43.6.632.

Estradiol Testing in the Exploration of Early Onset Puberty: Comparison of RIA and Chemiluminescence Immunoassay

• Blin J
• Hochman C
• Graveline N
• Masson D
• Baron S
• Bach-Ngohou K

• KMID: 2552043
• DOI: http://doi.org/10.3343/alm.2023.43.6.632

Figure

• 

  Fig. 1 Comparison between the RIA and DiaSorin assay (DA) for measuring estradiol (E2) levels. (A) Scatterplot of the comparison between the DA and RIA for all values (Pearson r=0.96). (B) Scatterplot of the comparison between the DA and RIA for values under 30 pg/mL (Pearson r=0.31). (C) Bland–Altman plot for all values. (D) Bland–Altman plot for values under 30 pg/mL.

• 

  Fig. 2 Estradiol (E2) serum levels in 41 young girls according to their clinical data (prepuberty, n=24; pubertal start, n=8; puberty, n=9) through RIA and the DiaSorin assay (DA). Dots represent individual data points, bars represent mean, and error bars represent standard errors of the mean.

Reference

1. Smy L, Straseski JA. 2018; Measuring estrogens in women, men, and children: recent advances 2012-2017. Clin Biochem. 62:11–23. DOI: 10.1016/j.clinbiochem.2018.05.014. PMID: 29800559.

2. Rosner W, Hankinson SE, Sluss PM, Vesper HW, Wierman ME. 2013; Challenges to the measurement of estradiol: an endocrine society position statement. J Clin Endocrinol Metab. 98:1376–87. DOI: 10.1210/jc.2012-3780. PMID: 23463657. PMCID: PMC3615207.

3. Owen LJ, Monaghan PJ, Armstrong A, Keevil BG, Higham C, Salih Z, et al. 2019; Oestradiol measurement during fulvestrant treatment for breast cancer. Br J Cancer. 120:404–6. DOI: 10.1038/s41416-019-0378-9. PMID: 30679781. PMCID: PMC6461991.

4. Taylor AE, Keevil B, Huhtaniemi IT. 2015; Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow. Eur J Endocrinol. 173:D1–12. DOI: 10.1530/EJE-15-0338. PMID: 25877990.

5. Desai R, Harwood DT, Handelsman DJ. 2019; Simultaneous measurement of 18 steroids in human and mouse serum by liquid chromatography-mass spectrometry without derivatization to profile the classical and alternate pathways of androgen synthesis and metabolism. Clin Mass Spectrom. 11:42–51. DOI: 10.1016/j.clinms.2018.12.003. PMID: 34841072. PMCID: PMC8620903.

6. Sehested A, Juul AA, Andersson AM, Petersen JH, Jensen TK, Müller J, et al. 2000; Serum inhibin A and inhibin B in healthy prepubertal, pubertal, and adolescent girls and adult women: relation to age, stage of puberty, menstrual cycle, follicle-stimulating hormone, luteinizing hormone, and estradiol levels. J Clin Endocrinol Metab. 85:1634–40. DOI: 10.1210/jcem.85.4.6512. PMID: 10770209.

7. Li M, Chen Y, Liao B, Tang J, Zhong J, Lan D. 2021; The role of kisspeptin and MKRN3 in the diagnosis of central precocious puberty in girls. Endocr Connect. 10:1147–54. DOI: 10.1530/EC-21-0182. PMID: 34414898. PMCID: PMC8494402.