Korean J Transplant.  2023 Nov;37(Suppl 1):S29. 10.4285/ATW2023.F-5966.

Expression of p53 and beta-catenin proteins in hepatocellular carcinoma

• Taivan N
• Ariya E
• Khurelsukh B
• Sondor G
• Ankhbayar E
• Gan-Erdene B
• Gantulga D
• Oyunsuren T

• KMID: 2551603
• DOI: http://doi.org/10.4285/ATW2023.F-5966

Abstract

The expression of p53 and beta-catenin proteins in hepatocellular carcinoma (HCC) samples with mutations in the tumor sup-pressor TP53 gene, which has a high frequency of mutations in liver cancer, and the CTNNB1 gene, which plays an important role in the Wnt signaling pathway, were identified. As a result, the beta-catenin protein was increased in 83.33% of liver tumor samples with CTNNB1 gene mutation, and p53 protein expression was increased in 50% of samples with TP53 gene mutation. According to this, it may be that liver tumors were caused by changes in p53 and beta-catenin protein expression. In the study, we selected 14 liver tumor samples and 14 adjacent nontumor samples with TP53 and CTNNB1 gene mutations and deter-mined the protein concentration based on the bicinchoninic acid method. A standard calibration curve was established with bovine serum albumin.