Mycobiology.  2023 Dec;51(6):388-392. 10.1080/12298093.2023.2282257.

Neodothiora pruni sp. nov., a Biosurfactant-Producing Ascomycetous Yeast Species Isolated from Flower of Prunus mume

• Kim JS
• Lee M
• Heo J
• Kwon SW
• Yun BS
• Kim Y

• KMID: 2550767
• DOI: http://doi.org/10.1080/12298093.2023.2282257

Abstract

A yeast strain, designated as JAF-11 ^T , was isolated from flower ofPrunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11 ^T was closely related to Neodothiora populina CPC 39399 ^T with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79 ^T with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequen ces of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11 ^T was well-separated from Neodothiora populina CPC 39399 ^T . In addition to the phylo genetic differences, strain JAF-11 ^T was distinguished from its closest species, Neodothiora populina CPC 39399 T and Rhizosphaera macrospora CBS 208.79 ^T belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11 ^T (KACC 48808 ^T ; MB 850034).

Keyword

Ascomycetous yeast; biosurfactant; novel yeast species